Biobanking in the Stem Cell Era

Biobanking in the Stem Cell Era
A Technical and Operational Guide

Jennifer C. Moore, Michael H. Sheldon, Ronald P. Hart
ISBN: 9781615044726 | PDF ISBN: 9781615044733
Copyright © 2012 | 86 Pages | Publication Date: 06/01/2012

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The study of mental health disorders and the genetics behind these disorders can be greatly enhanced by the use of induced pluripotent stem cells (iPSC). Since many mental health disorders develop after puberty, the only way in which to study the genetic mechanism of these diseases previously was through cellular surrogates, such as blood or cultured fibroblasts. Having the ability to reprogram adult cells to the pluripotent stage provides the capacity to study the onset of these disorders during a culture model of neural development and to include the impact of genetic risk factors and potential environmental triggers. Working with the National Institute of Mental Health (NIMH), the Rutgers Cell and DNA Repository (RUCDR) has begun banking iPSC source cells and converting those source cells into iPSC for distribution to the scientific community. Although initial protocols were developed to reprogram fibroblasts, the ability to reprogram blood cells has several advantages including less invasive collection, less post collection manipulation, and the large number of samples in existing collections. Here, we provide detailed protocols for reprogramming either fibroblasts with retroviral vectors or cryopreserved lymphocytes with Sendai viral vectors. Our goal is to support the discovery of effective treatments for mental health disorders.

Table of Contents

Acknowledgments
Introduction
Appendix
General Protocols
References
Author Biographies

About the Author(s)

Jennifer C. Moore, Department of Genetics and NIMH Stem Cell Center at the Rutgers University Cell & DNA Repository
Dr. Jennifer Moore is the Associate Director of the National Institute of Mental Health's (NIMH) Stem Cell Resource at Rutgers, the State University of New Jersey. Dr. Moore received her Bachelor's of Science degree from the University of North Carolina at Charlotte in Chemistry and her Ph.D. from the University of North Carolina at Chapel Hill in Biochemistry and Biophysics. At the NIMH Stem Cell Resource, Dr. Moore oversees the generation of iPSC and their differentiation into neural derivatives for the study of psychiatric disorders.

Michael H. Sheldon, Department of Genetics and NIMH Stem Cell Center at the Rutgers University Cell & DNA Repository
Dr. Michael Sheldon is the Director of the National Institute of Mental Health (NIMH) Stem Cell Resource at the Rutgers University Cell and DNA Repository (RUCDR), the largest academically based cell and DNA repository in the United States. Dr. Sheldon received his Bachelor of Arts degree from Cornell University, and a Ph.D. in Genetics from SUNY at Stony Brook. Dr. Sheldon oversees all operational aspects of the NIMH Stem Cell Resource, including the processing of tissue biopsies for cultivation of primary source cells for iPSC reprogramming.

Ronald P. Hart, Department of Cell Biology & Neuroscience, Rutgers University
Dr. Ronald Hart is a professor in the department of Cell Biology & Neuroscience at Rutgers University and also a member of the W.M. Keck Center for Collaborative Neuroscience, the Rutgers Stem Cell Research Center, and the Human Genetics Institute of New Jersey. He obtained a B.S. from the University of Connecticut and a Ph.D. from the University of Michigan Medical School. His postdoctoral training was at Rockefeller University. His group studies molecular mechanisms of stem cell differentiation into neurons, focusing on the roles of non-coding RNAs, specific transcription factors, and epigenetic signaling.

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